Quantitative Analysis in Organic Chemistry
Let’s explore how chemists measure elements in compounds! 🔍
🔥 Carbon & Hydrogen Estimation
Burn organic compound with excess oxygen & CuO:
Apparatus setup:
Organic compound → Combustion tube → U-tube (anhydrous CaCl₂ for H₂O) → U-tube (KOH for CO₂)
Calculations:
- Mass of compound = m g
- Mass of CO₂ produced = m₂ g
- Mass of H₂O produced = m₁ g
Example: 0.246g compound → 0.198g CO₂ & 0.1014g H₂O
%C = 21.95%, %H = 4.58% ✅
⛓ Nitrogen Estimation
Method 1: Dumas Method
Heat compound with CuO in CO₂ atmosphere → Measure N₂ gas
Example: 0.3g compound → 50mL N₂ at 300K & 700mm pressure → %N = 17.46%
Method 2: Kjeldahl Method
For -NH₂ and -CONH₂ groups only!
Compound + conc. H₂SO₄ → (NH₄)₂SO₄ → NH₃ gas absorbed in acid
Where M = acid molarity, V = acid vol, V₁ = NaOH vol for titration
Example: 0.5g compound → 10mL 1M H₂SO₄ neutralized → %N = 56% 🧪
🧪 Halogen Estimation (Carius Method)
Heat compound with fuming HNO₃ + AgNO₃ → Precipitate AgX
Example: 0.15g compound → 0.12g AgBr → %Br = 34.04%
💛 Sulphur Estimation
Compound + Na₂O₂/fuming HNO₃ → H₂SO₄ → BaSO₄ precipitate
Example: 0.157g compound → 0.4813g BaSO₄ → %S = 42.10%
📌 Phosphorus Estimation
Two methods:
- Precipitate as (NH₄)₃PO₄·12MoO₃:\% \text{P} = \frac{31 \times m_1 \times 100}{1877 \times m}
- Precipitate as Mg₂P₂O₇:\% \text{P} = \frac{62 \times m_1 \times 100}{222 \times m}
💨 Oxygen Estimation
%O = 100% – (sum of all other elements)
OR Direct method:
(where m₁ = mass of CO₂ produced from oxygen conversion)
⭐ NEET Super Shorts
- 🧪 Kjeldahl limitation: Doesn’t work for nitro (-NO₂), azo (-N=N-), or ring nitrogen (e.g., pyridine)
- ⚖️ Dumas vs Kjeldahl: Dumas for all N-compounds, Kjeldahl only for amino/proteins
- 💡 Sulphur test trick: Violet color with sodium nitroprusside = S present!
- 🔥 Combustion analysis: CaCl₂ absorbs H₂O, KOH absorbs CO₂
- 💧 Halogen test: Always decompose cyanides/sulphides first if N/S present!
Happy studying! 💯